Document Type : Original Article
Authors
1
Food Science and Dairy Technology. Faculty of Agriculture, South Valley University, 83523 Qena, Egypt
2
Agriculture Botany Department, Faculty of Agriculture, South Valley University, Qena, Egypt
3
Dairy Science Department, Faculty of Agriculture, Sohag University, Sohag, Egypt
Abstract
Identification of Bifidobacterial species still remain difficult and the chemical tests for identification of species of Bifidobacteria are now superseded by use of PCR. This study aimed to identify and examine some Bifidobacteria strains by chemical tests and not the method of PCR, identification of the bacterial strains was performed where the strains were initially tested for Gram stain, catalase test, production of Co2 (gas) from glucose, determined in Man, Rogosa, and Sharp (MRS) broth, as well as the ability to grow at different temperatures (5, 15, 25, 37° C) for 5 days, were considered to identify the strains. The strains were activated in sterile de Man, Rogosa, and Sharp broth inoculation and then incubated at 37° C for 7 days. The MRS broth was supplemented with various salt concentrations (4 % & 6.5%) of NaCl, to determine the ability of strains to grow at different concentrations of salt (NaCl). The results showed that Bifidobacterium is a genus of Gram positive, and the bacterial colonies on MRS agar are rod shapes, catalase negative, and it can’t produce gas from glucose. Strains of Bifidobacterium can grow in the temperature range from 5°C - 37°C, and Bifidobacterium species can’t tolerate the salt concentration.
Keywords
Main Subjects
)
View on SCiNiTO