In-vitro and in-vivo studies on the cell sap extract of some plant waste and using a natural biocidal to control the red mites Tetranychus urticae

Document Type : Original Article

Authors

Department of Agricultural Biochemistry, Faculty of Agriculture, Beni-Suef University, Beni-Suef, 62521, Egypt

Abstract

In the present work, three different samples of plant waste were studied; these are leaves of moringa, olive, and pomegranate trees. All three samples contain phenolic acids, phenols, and flavonoids. The total phenolic compounds (TPCs) in the samples were in moringa, olive, and pomegranate (780, 698, and 650 µg/g), respectively. HPLC-fractionation of phenolic compounds revealed the existence of 8 phenolic acids and 4 phenols. The most abundant acid is p < /em>- hydroxybenzoic acid and the levels are 490.2, 310.5, 300 µg/g, and the least abundant acid is ellagic acid (1.0, 20.01, and 5.60 µg/g). The highest concentrations of hydroxytyrosol are 190.04, 310.04, and 180.75 µg/g, and the levels of vanillin are 50.01, 24.20, and 30.19 µg/g sequentially samples and found 14 compounds is a flavonoid. And when those extracts were tested at four concentrations (2.5, 5, 7.5, and 10 ml/L). The results obtained from the use of natural pesticides understudy at the previously mentioned concentrations were as follows (35, 57, 73, and 91 %) moringa extract, (19, 30, 43, and 59%) olive extract, and (33, 45, 65, and 72 %) pomegranate extract with the use of a standard chemical pesticide (Vertimec 1.8%) against mites, the result obtained from it was 33% of insects. The results of the in-vitro experiment were confirmed by an in-vivo applied field experiment

Keywords

Main Subjects