SRAP Molecular Markers Linked to Three Morphological Traits in Egyptian Bread Wheat (Triticum aestivum L.)

Khaled, A.G.A.; Elameen, T.M.; Ahmed, A.Y.M.; Mohiy, M.; Elshazly, I.F.O.

doi:10.21608/svuijas.2021.78975.1113

SRAP Molecular Markers Linked to Three Morphological Traits in Egyptian Bread Wheat (Triticum aestivum L.)

Document Type : Original Article

Authors

¹ Department of Genetics, Faculty of Agriculture, Sohag University, Sohag, Egypt.

² Department of Genetics, Faculty of Agriculture, South Valley University, Qena, 83523, Egypt.

³ Wheat Res. Dep., Field Crops Res. Inst., ARC, Giza, Egypt.

10.21608/svuijas.2021.78975.1113

Abstract

Sequence Related Amplified Polymorphism (SRAP) molecular technique was used for assessing the genetic diversity of eight Egyptian bread wheat (Triticum aestivum L.) genotypes. Five SRAP primers combinations were able to expose the polymorphism between the studied genotypes. The percentage of polymorphism (P %) ranged from 40 to 79.90 %. Furthermore, the Polymorphic Information Content (PIC) values for SRAP primers combination varied from 0.11 to 0.31 with an average of 0.19. The ME5F-EM2R and ME1F-EM2R primer combination cleared higher levels of polymorphism of 66.67 and 76.90%, respectively. The results of Single Marker Analysis (SMA) showed that the SRAP marker ME1-EM21320bp could be considered probably as candidate marker linked to spike length in the tested genotypes. Moreover, ME1-EM2900bp and ME1-EM2575bp could be considered as markers linked to plant height and tillers number /plant traits, respectively. Results of Single Marker Analysis (SMA) showed significant marker-trait associations for spike length (P= 0.04), plant height (P= 0.02) and number of tillers/plant (P= 0.03). Me1F-Em6R850bp marker could be also considered as candidate markers probably linked to plant height, with a specific fragment of 850bp for the tolerant genotype (P1). UPGMA cluster analysis based on SRAP markers separated the studied wheat genotypes into three significantly different clusters. The dendrogram based on morphological traits divided the studied wheat genotypes into two different clusters. The Mantel test revealed that there was a positive but non-significant correlation between the genetic similarities based on phenotypic data and SRAP marker (r = 0.36, P> 0.05).

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